ABSTRACT
It has been shown that both multidrug resistance-associated protein (MRP1) and canalicular
multi-specific organic anion transporter (cM0AT/MRP2) have the ability to extrude
glutathione conjugates (GS-X pump activity) from cells. Therefore, they play an important
role in the detoxification of xenobiotics. Using mrpl-knockout mice, it has recently
been shown that MRPl/mrpl has an important role in the export of leukotriene C4 (LTC4, a mediator of inflammation, and in protecting the body from a number of toxins,
including several antitumor drugs. A comparison of the transport properties across
the bile canalicular membrane in normal and mutant rats, whose cMOAT function is hereditarily
defective, has shown that the physiologic role of cMOAT is to excrete LTC4, bilirubin glucuronides, 17β-estradiol-17β-D-glucuronide, and reduced folates. In
the present review article, we summarize the substrate specificity and mechanism for
the transport of these GS-X pumps, focusing on the pharmacologic and physiologic aspects.
The transport activity mediated by cMOAT is also discussed in terms of a comparison
between membrane vesicles from hepatocytes and cMOAT-transfected cells, and we also
briefly examine the possible role of MRP I and cMOAT in the extrusion of reduced glutathione.
KEY WORDS
multidrug resistance-associated protein - canalicular multispecific organic anion
transporter - glutathione conjugate pumps - ATP-binding cassette transporter - biliary
excretion - glutathione